|
|
|
|
|
| WISBECK,ELISABETH; FACCHINI,JEAN M.; ALVES,ENDI P.; SILVEIRA,MARCIA L.L.; GERN,REGINA M.M.; NINOW,JORGE L.; FURLAN,SANDRA A.. |
| ABSTRACT Fungi of Pleurotus genus have attracted a great interest due to their medicinal properties such as anti-inflammatory, antimicrobial and antitumor. These properties are attributed mainly to polysaccharides synthesized by Pleurotus. This work aimed to study the mycelial growth of P. ostreatus in submerged culture, evaluating the influence of the initial concentration of substrate (20 and 40 g/L of glucose) and the pH (4 and 6) on kinetic parameters of production of biomass. The effectiveness of different doses (10, 30 and 50 mg/kg) of a mycelium polysaccharide fraction extracted from P. ostreatus in reducing Sarcoma 180 development in mice was also verified. In the range of this study, maximum concentration of mycelial biomass (about 12.8 g/L) was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antitumor Activity; Mycelium; Pleurotus ostreatus; Polysaccharides; Sarcoma 180. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502013 |
| |
|
|
| FERREIRA,PAULO MICHEL PINHEIRO; COSTA,PATRICIA MARÇAL DA; COSTA,ARINICE DE MENEZES; LIMA,DAISY JEREISSATI BARBOSA; DRUMOND,RENATA ROSADO; SILVA,JURANDY DO NASCIMENTO; MOREIRA,DIOGO RODRIGO DE MAGALHÃES; OLIVEIRA FILHO,GEVÂNIO BEZERRA DE; FERREIRA,JAMILE MAGALHÃES; QUEIROZ,MARIA GORETTI RODRIGUES DE; LEITE,ANA CRISTINA LIMA; PESSOA,CLÁUDIA. |
| Eleven phthalimide derivatives were evaluated with regards to their antiproliferative activity on tumor and normal cells and possible toxic effects. Cytotoxic analyses were performed against murine tumors (Sarcoma 180 and B-16/F-10 cells) and peripheral blood mononuclear cells (PBMC) using MTT and Alamar Blue assays. Following, the investigation of cytotoxicity was executed by flow cytometry analysis and antitumoral and toxicological potential by in vivo techniques. The molecules 3b, 3c, 4 and 5 revealed in vitro cytotoxicity against Sarcoma 180, B-16/F-10 and PBMC. Since compound 4 was the most effective derivative, it was chosen to detail the mechanism of action after 24, 48 and 72 h exposure (22.5 and 45 µM). Sarcoma 180 cells treated with compound 4... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytotoxicity; Histological alterations; Murine cells; Phthalimide derivatives; Sarcoma 180. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100313 |
| |
|
| |
|
|
|
